Manuka Honey

Manuka Honey

Manuka Honey is a unique honey from New Zealand renowned for its special properties which include the presence of the naturally occurring compound methylglyoxal. Research continues to uncover the many attributes of Manuka Honey.

Manuka honey is bactericidal against Pseudomonas aeruginosa and results in differential expression of oprF and algD

Aled E L Roberts, Sarah E Maddocks and Rose A Cooper


The presence of Pseudomonas aeruginosa in surface wounds is of clinical significance and can lead to persistent infections. Manuka honey has gained ground in clinical settings due to its effective therapeutic action and broad spectrum of antibacterial activity. In this study, the effect of manuka honey on P. aeruginosa was investigated using MIC, MBC, growth kinetics, confocal microscopy, atomic force microscopy and real-time PCR. A bactericidal mode of action for manuka honey against P. aeruginosa was deduced (12% w/v MIC; 16% w/v MBC) and confirmed by confocal and atomic for microscopy, which showed extensive cell lysis after 60 minutes exposure to inhibitory concentrations of manuka honey. The inability of honey treated cells to form micro-colonies was demonstrated and investigated using Q-PCR for three key micro-colony forming genes: algD, lasR, and oprF. algD expression increased 18-fold whereas oprF expression decreased 8-fold following honey treatment; lasR expression remained unaltered. These findings confirm that manuka honey is effective at inducing cell lysis and identifies two targets, at the genetic level, that might be involved in this process.

From Microbiology